The Biomolecule Analysis Core Facility (BACF) specializes in the separation and characterization of biomolecules. The facility possesses state-of-the-art equipment for proteomic analysis and the characterization of molecular binding interactions. These resources promote new insight into the mechanism of infectious agents, cancer, and other diseases that affect the population of the Paseo del Norte region. BACF services are available to external collaborators for a small fee.
Spectrophotometer available for use by registered users
Benchtop ultracentrifuge available for use by registered users.
"The Biacore T100 uses Surface Plasmon Resonance (SPR) technology to detect sensitive and reliable characterization of molecular binding interactions in real-time. The SPR technology monitors the changes of mass bound to the surface of the chip, providing sensitive characterization of biomolecular interactions in terms of specificity, kinetics and affinity.
The Biacore T100 is run exclusively by a dedicated operator."
Centrivap available for use by registered users.
Lyophilizer available for use by registered users.
Tunable Microplate Reader-Spectrophotometer - 96 well plate format.
Available for use by registered users.
Gamma counter available for use by registered users.
Liquid scintillation counter available for use by registered users.
Sonicator available for use by registered users
"A Thermo Polaris Q ion-trap mass spectrometer equipped with a Trace GC Ultra gas chromatograph with a split/splitless injector for mass spectrometric identification of the GC components and quantitative composition analyses."
"A Thermo LTQXL/ETD MS ESI-linear ion trap-MS with electron-transfer dissociation (ETD) capability coupled to a nanoLC-1D Plus from Eksigent for analyses of minute amounts (picomoles to femtomoles) of sample.
The instrument is equipped at the front end with a TriVersa NanoMate electrospray ionization nano-source."
Fluorometer available to registered users.
Luminometer available to registered users.
Low speed centrifuge available for use by registered users.
"- The Sorvall® RC-6 Plus superspeed centrifuge is a compact, user-friendly design that speeds up to 22,000 rpm (55,200 xg). The RC-6 Plus is a high-productivity solution that delivers maximum throughput and minimum maintenance.
- The RC-6 centrifuge is operated on a code basis, which is provided after orientation."
"Available with the following rotors:
- F13S-14x50cy: can spin 14 x 50 ml conical tubes up to 26,727 x g, reducing processing time and eliminating the need for sample transfers. Max.Speed RPM 13000 Max. g-force 28960
- F14S-6x250y: The Fiberlite F14S-6x250y carbon fiber rotor can be paired with Nalgene 250 ml Oak Ridge bottles for quickly and safely processing a wide range of laboratory protocols, from basic separations to cutting edge research. Max. Speed14000 Max. g-force 30100
- F21S-8x50y: The Fiberlite F21-8x50y carbon fiber rotor can be paired with 50 mL Nalgene Oak Ridge Polypropylene or Oak Ridge Polycarbonate bottles with sealing cap. Max. Speed RPM 21000 Max. g-force 52356"
"- Thermo Scientific* Sorvall WX Ultra Series floor standing laboratory centrifuge combines speed and powerful separation in a quiet compact, energy-efficient design. Available in 80,000 rpm model.
- The RC-6 centrifuge is operated on a code basis, which is provided after orientation.
- Available with the following rotors:
- T-865: Capacity 8 x 36 Max. Speed RPM 65,000 Max g-force 429,460
- AH-650: Capacity 6 x 5 Max. Speed RPM 50,000 Max g-force 296,010
- TH-641: Capacity 6 x 13.2 Max. Speed RPM 41,000 Max g-force 287,660"
Bath sonicator available for use by registered users.
HPLC available for use by registered users.
"New users must undergo a workshop/training session given by the coordinator before using any shared (open access) equipment. Training session(s) should be scheduled with the coordinator."
"For molecular mass determination, one needs approx. 1-10 picomoles of a purified peptide or protein. If the sample contains non-volatile salt, Sep-Pak C18 purification needs to be performed prior to MS analysis.
For peptide and protein sequencing and identification from SDS-PAGE gel bands, any band visible by Coomassie staining is acceptable (roughly corresponding to a minimum peptide or protein concentration in the range of 1-0.1 pmol/µl). You may also combine up to 3 duplicate gel bands in one single microtube to send us for analysis. This way you will increase your chances to get one or more peptide sequences and you're your protein identified."